Stratus made her debut on the March 19, 2000 episode of Sunday Night Heat, under the ring name Trish Stratus. She appeared on stage to scout WWF Superstars, in particular Test and Prince Albert. The next night on Raw Stratus began her first role in the company, managing Test and Albert in the tag team T & A. It was during her stint managing T & A that Stratus took her first major bump in the ring, being driven through a table by the Dudley Boyz at Backlash after she had been taunting Bubba Ray Dudley for several weeks. She also began managing Val Venis to win the Intercontinental Championship but their partnership ended at SummerSlam after Venis lost the title.

Stratus made her in-ring debut on the June 20 taping of SmackDown!, winning a tag team match with T & A against the Hardy Boyz and Lita. A storyline feud between Stratus and Lita developed after the match with Stratus attacking Lita on episodes of Raw and SmackDown!, leading to an Indian Strap match on the J